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Figure 3. Effect of enriching drinking water with L-Cit or hydrolyzed soy protein (=Iso-N-control) on markers of senescence in 4-, 16-, and 20-month-old C57BL/6J mice. (a) p16 protein concentration in the western blot of plasma; (b) total PAI-1 concentration in plasma; <t>(c)</t> <t>IL6</t> concentration in the western blot of plasma; (d) <t>CRP</t> concentration in the western blot of plasma; and (e) representative images of p16, CRP, and IL6 and corresponding Ponceau images. Representative images of full- size membranes of western blots are shown in Supplemental Figures S1–S5. Data are presented as means ± SEM, * p ≤0.05. CRP, C-reactive protein; IL6, interleukin 6; Iso-N-control, hydrolyzed soy protein; L-Cit, L-Citrulline; PAI-1, plasminogen activator inhibitor-1.
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Figure 3. Effect of enriching drinking water with L-Cit or hydrolyzed soy protein (=Iso-N-control) on markers of senescence in 4-, 16-, and 20-month-old C57BL/6J mice. (a) p16 protein concentration in the western blot of plasma; (b) total PAI-1 concentration in plasma; <t>(c)</t> <t>IL6</t> concentration in the western blot of plasma; (d) <t>CRP</t> concentration in the western blot of plasma; and (e) representative images of p16, CRP, and IL6 and corresponding Ponceau images. Representative images of full- size membranes of western blots are shown in Supplemental Figures S1–S5. Data are presented as means ± SEM, * p ≤0.05. CRP, C-reactive protein; IL6, interleukin 6; Iso-N-control, hydrolyzed soy protein; L-Cit, L-Citrulline; PAI-1, plasminogen activator inhibitor-1.
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Figure 3. Effect of enriching drinking water with L-Cit or hydrolyzed soy protein (=Iso-N-control) on markers of senescence in 4-, 16-, and 20-month-old C57BL/6J mice. (a) p16 protein concentration in the western blot of plasma; (b) total PAI-1 concentration in plasma; <t>(c)</t> <t>IL6</t> concentration in the western blot of plasma; (d) <t>CRP</t> concentration in the western blot of plasma; and (e) representative images of p16, CRP, and IL6 and corresponding Ponceau images. Representative images of full- size membranes of western blots are shown in Supplemental Figures S1–S5. Data are presented as means ± SEM, * p ≤0.05. CRP, C-reactive protein; IL6, interleukin 6; Iso-N-control, hydrolyzed soy protein; L-Cit, L-Citrulline; PAI-1, plasminogen activator inhibitor-1.
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Figure 3. Effect of enriching drinking water with L-Cit or hydrolyzed soy protein (=Iso-N-control) on markers of senescence in 4-, 16-, and 20-month-old C57BL/6J mice. (a) p16 protein concentration in the western blot of plasma; (b) total PAI-1 concentration in plasma; <t>(c)</t> <t>IL6</t> concentration in the western blot of plasma; (d) <t>CRP</t> concentration in the western blot of plasma; and (e) representative images of p16, CRP, and IL6 and corresponding Ponceau images. Representative images of full- size membranes of western blots are shown in Supplemental Figures S1–S5. Data are presented as means ± SEM, * p ≤0.05. CRP, C-reactive protein; IL6, interleukin 6; Iso-N-control, hydrolyzed soy protein; L-Cit, L-Citrulline; PAI-1, plasminogen activator inhibitor-1.
C Reactive Protein Crp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 3. Effect of enriching drinking water with L-Cit or hydrolyzed soy protein (=Iso-N-control) on markers of senescence in 4-, 16-, and 20-month-old C57BL/6J mice. (a) p16 protein concentration in the western blot of plasma; (b) total PAI-1 concentration in plasma; (c) IL6 concentration in the western blot of plasma; (d) CRP concentration in the western blot of plasma; and (e) representative images of p16, CRP, and IL6 and corresponding Ponceau images. Representative images of full- size membranes of western blots are shown in Supplemental Figures S1–S5. Data are presented as means ± SEM, * p ≤0.05. CRP, C-reactive protein; IL6, interleukin 6; Iso-N-control, hydrolyzed soy protein; L-Cit, L-Citrulline; PAI-1, plasminogen activator inhibitor-1.

Journal: Biomolecules

Article Title: Supplementing L-Citrulline Can Extend Lifespan in C. elegans and Attenuate the Development of Aging-Related Impairments of Glucose Tolerance and Intestinal Barrier in Mice.

doi: 10.3390/biom13111579

Figure Lengend Snippet: Figure 3. Effect of enriching drinking water with L-Cit or hydrolyzed soy protein (=Iso-N-control) on markers of senescence in 4-, 16-, and 20-month-old C57BL/6J mice. (a) p16 protein concentration in the western blot of plasma; (b) total PAI-1 concentration in plasma; (c) IL6 concentration in the western blot of plasma; (d) CRP concentration in the western blot of plasma; and (e) representative images of p16, CRP, and IL6 and corresponding Ponceau images. Representative images of full- size membranes of western blots are shown in Supplemental Figures S1–S5. Data are presented as means ± SEM, * p ≤0.05. CRP, C-reactive protein; IL6, interleukin 6; Iso-N-control, hydrolyzed soy protein; L-Cit, L-Citrulline; PAI-1, plasminogen activator inhibitor-1.

Article Snippet: Membranes were incubated with primary antibodies for p16 (Biorbyt Ltd., Cambridge, UK), intestinal fatty acid binding protein (IFABP, Abcam, Cambridge, UK), IL6 (Santa Cruz Biotechnology, Inc., Heidelberg, Germany), and CRP (Santa Cruz Biotechnology, Inc., Heidelberg, Germany), and corresponding HRPlinked secondary antibodies.

Techniques: Control, Protein Concentration, Western Blot, Clinical Proteomics, Concentration Assay